Synthesis of [Phe(4F)3]thymopoietin II and examination of its immunological effect on the impaired blastogenic response of T-lymphocytes of uremic patients
✍ Scribed by Takashi Abiko; Hiroshi Sekino
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 585 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0968-0896
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✦ Synopsis
Abstraet--[Phe(4F)3]thymopoietin lI was synthesized using a conventional solution method. The depmtection of the protected [Phe(4F)3]thymopoietin II was achieved by treatment with 1 M trifluoromethanesulfonic acid:thioanisole (molar ratio 1:1) in trifluoroacetic acid in the presence of dimethylselenide and m-cresol. The synthetic [Phe(4F)3]thymopoietin II and thymopoietin II were tested for effect on impaired T-lymphocyte transformation by phytohemagglutinin in uremic patients suffering from recurrent infectious diseases. The restoring activity on the impaired phytohemagglutinin stimulation of T-lymphocytes was obtained after incubation of peripheral lymphocytes isolated from uremic patients with the synthetic [Phe(4F) 3 ]thymopoietin 1]. This peptide exhibited far stronger restoring effect than that of our synthetic thymopoietin II.