The validity of in vivo fluorometric assays was ascertained for phage and bacterial DNA measurements. The following parameters were determined by this simple technique. The DNA content of dividing cells of Bacillus subtilis 168/2 was 2.65 times higher than in resting cells. Assuming that resting cel
Synthesis of phage 2C-DNA in permeabilized B. subtilis
β Scribed by Hoet, Philippe ;Coene, Marc ;Cocito, Carlo
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 639 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The development of bacteriophages SPP1 and phi 29 has been studied in several B. sutilis mutants defective in host DNA replication, under non permissive conditions. Several gene products, involved in the synthesis of host DNA, are required for phi 29 replication, while SPP1 seems to require only the
Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h
Phage SPP1 infecting a mutator strain of B. subtilis (BD337) which carries a defective DNA polymerase III is mutagenized. This effect is absent in phages SPO2, SP82G and ΓΈe. The results confirm previous observations that SPP1 uses host DNA polymerase III for its DNA synthesis.