Synthesis of peptides onto the surface of poly(ethylene terephthalate) particle track membranes

✍ Scribed by Alexander Papra; Hans-Georg Hicke; Dieter Paul

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 146 KB
• Volume: 74
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/(sici)1097-4628(19991114)74:7<1669::aid-app9>3.0.co;2-w

✦ Synopsis

In this article, we describe the synthesis of polypeptides onto the whole specific surface of poly(ethylene terephthalate) particle track membranes. In this case, initial functionalization is necessary. It had been done by an optimized surface oxidation in accordance with the method of Marchant-Brynaert, J.; Deldime, M.; Dupont, I.; Dewez, J.-L.; Schneider, Y.-J. (J Colloid Interface Sci 1995, 173, 236). Subsequent activation with carbodiimide and reaction with multifunctional amines yielded an aminated surface. The synthesis of peptides was carried out by two different means: first, by the coupling of single amino acids analogous to the method of Merrifield, and second, by coupling of presynthesized polypeptides consisting of 18 helix-forming amino acids. Analytical characterization was carried out by fluorescence spectroscopy by using the label Fluram and confirmed by the results of X-ray photoelectron (XP) spectroscopy. Only the stepwise synthesis led to a dense surface functionalization with peptides, whereas the coupling of fragments resulted in lower yields of coupling.