Protease-catalyzed peptide synthesis usi
β
Volker Schellenberger; Ute Schellenberger; Hans-Dieter Jakubke; Nina P. Zapevalo
π
Article
π
1991
π
John Wiley and Sons
π
English
β 296 KB
π 1 views
Benzyloxycarbonyl-L-proline p-guanidinophenyl ester is an "inverse substrate" for trypsin; i.e., the cationic center is included in the leaving group instead of being in the acyl moiety. This substrate can be used in trypsin-catalyzed acyl-transfer reactions leading to the sythesis of Pro-Xaa peptid