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Synthesis of peptide bonds by proteinases. Addition of organic cosolvents shifts peptide bond equilibriums toward synthesis

✍ Scribed by Homandberg, Gene A.; Mattis, Jeffrey A.; Laskowski, Michael


Book ID
126436325
Publisher
American Chemical Society
Year
1978
Tongue
English
Weight
976 KB
Volume
17
Category
Article
ISSN
0006-2960

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Protease-catalyzed peptide synthesis usi
✍ Volker Schellenberger; Ute Schellenberger; Hans-Dieter Jakubke; Nina P. Zapevalo πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 296 KB πŸ‘ 1 views

Benzyloxycarbonyl-L-proline p-guanidinophenyl ester is an "inverse substrate" for trypsin; i.e., the cationic center is included in the leaving group instead of being in the acyl moiety. This substrate can be used in trypsin-catalyzed acyl-transfer reactions leading to the sythesis of Pro-Xaa peptid