Synthesis of Organoselenium-Modified β-Cyclodextrins Possessing a 1,2-Benzisoselenazol-3(2H)-one Moiety and Their Enzyme-Mimic Study
✍ Scribed by Yu Liu; Bin Li; Li Li; Heng-Yi Zhang
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- German
- Weight
- 182 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0018-019X
No coin nor oath required. For personal study only.
✦ Synopsis
Six novel H 2 O-soluble b-cyclodextrin derivatives containing a 1,2-benzisoselenazol-3(2H)-one moiety were synthesized by a convenient method in 25 ± 60% yield and characterized by MS, elemental analysis, IR, 1 H-NMR, and UV/VIS spectroscopy. The conformations of these b-cyclodextrin derivatives 1 ± 6 were analyzed by circular dichroism and fluorescence-lifetime experiments. The superoxide dismutase (SOD) activities of 1 ± 6 were determined by auto-oxidation of pyrogallol at 25.08 in buffer solution (pH 8.2), giving relatively high SOD activities of up to 121 ± 330 U/mg. Also, the glutathione peroxidase (GPX) activities of hosts 1 ± 6, determined by the method of Wilson at 378 in buffer solution (pH 7.0), show good GPX activities in the range of 0.34 ± 0.86 U/mmol. The mimicking results of the bifunctional artificial enzyme models 1 ± 6 were globally compared with regard to their structural and conformational difference.