We have used M13 single-stranded DNA bound by uv to small pieces of nylon membrane for the synthesis of biotinylated single-stranded DNA probes. The labeling method requires a large fragment of DNA polymerase I and random hexanucleotides. There is no need for previous linearization of the template.
β¦ LIBER β¦
Synthesis of nucleic acid probes on membrane supports: A procedure for the removal of unincorporated precursors
β Scribed by Suraj P. Bhat
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 765 KB
- Volume
- 184
- Category
- Article
- ISSN
- 0003-2697
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