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Synthesis of lipids in mouse brain cell cultures during development

✍ Scribed by Dr. Hans Peter Siegrist; Liane Bologa-Sandru; Thomas Burkart; Ulrich Wiesmann; Karel Hofmann; Norbert Herschkowitz

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
418 KB
Volume
6
Category
Article
ISSN
0360-4012

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Several metabolic activities in dissociated cultures of newborn mouse brain were compared to the situation in vivo. The developmental activity pattern of cerebroside‐sulfotransferase, cyclic nucleotide phosphohydrolase, and β‐hydroxy‐β‐methyl glutaryl‐coenzyme A‐reductase and the synthesis and deposition of sulfatide and cholesterol in culture were estimated. The enzyme activity patterns in vivo and in culture are the same. Since the cultures show very little myelin formation, the parallel increase of enzyme activities necessary for myelination in vivo and in culture suggest the existence of intrinsic factors regulating the biochemical differentiation. In addition, the formation of the products, determined in culture, follows the patterns of the enzyme activities. Dissociated brain cell cultures are therefore a valid model for the study of biochemical parameters related to the synthesis of brain lipids during development.

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