Synthesis of linear and comb-like peptide constructs containing up to four copies of a T cell epitope and their capacity to stimulate T cells
✍ Scribed by Jürgen Mack; Kirsten Falk; Olaf Rötzschke; Tilmann Walk; Jack L. Strominger; Günther Jung
- Book ID
- 105360140
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 473 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1075-2617
- DOI
- 10.1002/psc.327
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✦ Synopsis
Abstract
Polypeptide constructs containing up to four copies of the T cell epitope 306‐318 of influenza virus haemagglutinin have been synthesized on solid phase. Between the copies, a non‐natural PEG‐based spacer amino acid has been introduced. The oligomeric epitopes were analysed by RP‐HPLC and ES‐MS. The arrangement of the epitopes within the peptide constructs was either linear or comb‐like. The proliferative response in a T helper cell assay induced by these oligomerized epitopes has been tested, showing that the linearly arranged epitopes are more effective than the comb‐like oligomers. Copyright © 2001 European Peptide Society and John Wiley & Sons, Ltd.