Synthesis of L-tyrosine glyceryl ester catalyzed by α-chymotrypsin in water-miscible organic solvents: A possible sun-tan accelerator product
✍ Scribed by Pedro Lozano; Didier Combes; José L. Iborra; Arturo Manjón
- Publisher
- Springer Netherlands
- Year
- 1993
- Tongue
- English
- Weight
- 383 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
The synthesis of L-tyrosine glyceryl ester, from glycerol and L-tyrosine methyl ester, was carried out by a transesterification reaction catalyzed by a-chymotrypsin. Values of 60 % (v/v) for glycerol and 200 mM for L-tyrosine methyl ester were optimal for the transesterification reaction. Additionally to glycerol, several other water miscible cosolvents (acetonitrile, N,N'-dime@ formamide and tetrahydrofurane) were tested in the reaction media, but their presence did not give an enhancement on the transesterification activity with respect to the glycerol/water medium. However, increasing the hydrophobic@ of the cosolvent resulted in a reduction of the enzyme activity, the water:glycerol mixture being the best reaction media.