Synthesis of human rotavirus polypeptides in cell culture

✍ Scribed by R. L. Heath; C. J. Birch

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 794 KB
• Volume: 25
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890250113

✦ Synopsis

The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of infection of the virus, incorporation of actinomycin D and excess NaCl into the medium resulted in significant reduction in host cell background and enabled observation of viral polypeptides as early as 2 hours post infection. Five polypeptides were found to be structural components of the virion, and a further eight appeared to be nonstructural proteins or intermediates. Five plypeptides were glycosylated during virus replication, but only one of these, VP7, was a definite structural glycoprotein. Pulse-chase experiments revealed that four low molecular weight polypeptides underwent posttranslational modifications.