✦ LIBER ✦
Synthesis of glucose 1-phosphate-U-14C and specifically labeled sucroses using sucrose phosphorylase
✍ Scribed by Bruce M. Chassy; Micah I. Krichevsky
- Publisher
- Elsevier Science
- Year
- 1972
- Tongue
- English
- Weight
- 500 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
More than twenty years ago Hassid, Doudoroff, and Barker investigated the mechanism of action of sucrose phosphorylase (disaccharide glucosyltransferase (non-specific), EC 2.4.1.7) isolated from Pseudomonas sac-c~a~o~~~~ using radioisotopes (l-3). These classical studies not only led to the postulation of the following mechanism:
(1) sucrose + enzyme -+ glucosyl-enzyme + fructose,
(2) glu~syl-enzyme