Synthesis of ethyl [14CH3]methylmalonyl thioglycolate as a possible substrate analogue of [14CH3]methylmalonyl coenzyme-A

✍ Scribed by I. Kovács; Z. Kovács

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 373 KB
• Volume: 29
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580291102

✦ Synopsis

Abstract

Ethyl methylmalonyl thioglycolate is a potential substrate analogue of methylmalonyl‐coenzyme‐A (methylmalonyl‐CoA) in the investigation of propionic acid metabolism. To prove this hypothesis, the tracer ethyl [^14^CH~3~]methylmalonyl thioglycolate was synthesized via methyl‐Meidrum's acid to carry out the biochemical examinations. The method described can also be used to synthesize [^14^CH~3~]methylmalonyl‐CoA by transesterification of active labelled methylmalonyl thiophenyl ester. This latter intermediate is chemically stable when stored at room temperature, and the unstable [^14^CH~3~]methylmalonyl‐CoA can be prepared in one step just preceeding the biochemical experiments.