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Synthesis of dolichyl phosphate derivatives with fluorescent label at the ω-end of the chain, new tools to study protein glycosylation

✍ Scribed by Vladimir N Shibaev; Vladimir V Veselovsky; Antonina V Lozanova; Sergei D Maltsev; Leonid L Danilov; W.Thomas Forsee; Jun Xing; Herbert C Cheung; Mark J Jedrzejas


Book ID
104364944
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
126 KB
Volume
10
Category
Article
ISSN
0960-894X

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✦ Synopsis


AbstractÐDerivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene ¯uorophore groups at the o-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.


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ChemInform Abstract: Derivatives of Doli
✍ V. V. Veselovsky; A. V. Lozanova; S. D. Maltsev; L. L. Danilov; V. N. Shibaev; M 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 28 KB 👁 1 views

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