Synthesis of basement membrane by gastrointestinal cancer cell lines

✍ Scribed by Akashi, Takumi; Miyagi, Tizuko; Ando, Noboru; Suzuki, Yoshimi; Nemoto, Tetsuo; Eishi, Yoshinobu; Nakamura, Kyoichi; Shirasawa, Takuji; Osa, Natsumi; Tanaka, Noboru; Burgeson, Robert E.

• Book ID: 102648479
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 529 KB
• Volume: 187
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199901)187:2<223::aid-path219>3.0.co;2-b

✦ Synopsis

Gastrointestinal adenocarcinoma-derived cell lines were studied in order to determine their pattern of expression of basement membrane components and their ability to form a basement membrane. In contrast to the well-preserved expression of laminin beta 2, beta 3, gamma 1, and gamma 2 chain mRNAs, five of eight gastrointestinal cancer cells lacked alpha 3 mRNA. Immunohistochemical and electron microscopic examination of four cell lines transplanted subcutaneously to SCID mice demonstrated the presence of both alpha 3 and alpha 5 chains and the formation of a basal lamina in two cases. The other two cell lines lacked both alpha 3 and alpha 5 chains and could not form a basal lamina, suggesting that this deficiency may be a factor which affects their ability to form a basement membrane. This abnormality might play some role in stromal invasion by tumour cells in gastrointestinal cancer.