✦ LIBER ✦
Synthesis of a water-soluble serine-based neoglycolipid which can be covalently linked to solid phases
✍ Scribed by Mikael Elofsson; Johan Broddefalk; Tomas Ekberg; Jan Kihlberg
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 813 KB
- Volume
- 258
- Category
- Article
- ISSN
- 0008-6215
No coin nor oath required. For personal study only.
✦ Synopsis
NO-Fmoc-serine pentafluorophenyl ester was glycosylated with perbenzoylated lactosyl bromide. The resulting product was coupled to a resin functionalized with 6aminohexanoic acid and then Na-acylated to give a serine-based analogue of lactosylceramide.
The water-soluble neoglycolipid was covalently linked to microtiter plates via its carboxyl group and was recognized by a lactose-binding lectin in an ELISA.