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Synthesis of a spin-labeled analog of nicotinamide adenine dinucleotide phosphate and its interaction with dihydrofolate reductase

โœ Scribed by Cocco, Lennie; Blakley, Raymond L.


Book ID
126973492
Publisher
American Chemical Society
Year
1979
Tongue
English
Weight
690 KB
Volume
18
Category
Article
ISSN
0006-2960

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## Abstract Uniformly labeled (U) ^14^C nicotinamide adenine dinucleotide phosphate (NADP^+^) was synthesized by phosphorylating [Uโ€^14^C]nicotinamide adenine dinucleotide (NAD^+^) in the presence of immobilized NAD^+^ kinase. The 15 ฮผCi (600 ฮผL) synthesis consistently achieved yields between 80% a