Synthesis of a polynucleotide corresponding to the promoter region of bacteriophage fd DNA
โ Scribed by Yu.A. Ovchinnikov; V.A. Efimov; O.G. Chakhmakcheva
- Book ID
- 115909600
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 560 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0014-5793
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Promotor sequences recognized by Escherichia coli RNA polymerase have been isolated from bacteriophage T7 DNA using the plasmid pBRH4. T7 DNA was digested with the restriction endonuclease Hae III, Alu I, and Eco RI\* and the products of these digestions were ligated into the EcoRI site of pBRH4. Cl
Derivatives of bacteriophages fd which transduce kanamycin resistance were selected after growth of the phage in an E. coli strain that carried transpoon 5 (Tn5). Different clones of transducing phage and their DNAs were characterized by gel electrophoresis, electron microscopy, and by their ability