Synthesis of a New Template with a Built-in Adjuvant and Its Use in Constructing Peptide Vaccine Candidates Through Polyoxime Chemistry
✍ Scribed by Weiguang Zeng; David C. Jackson; Keith Rose
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 554 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1075-2617
- DOI
- 10.1002/psc.51
No coin nor oath required. For personal study only.
✦ Synopsis
Synthetic lipopeptides are showing promise as vaccine candidates, but until now it has been very difficult to prepare them in homogeneous form. We describe the synthesis and characterization of a new water-soluble, four-branched template with a built-in lipophilic adjuvant (Pam3Cys). Through the use of oxime chemistry, we attached four copies of an unprotected influenza virus peptide and characterized the product (13 kDa) by reversed-phase HPLC and electrospray ionization mass spectrometry. Several other such constructions were made using the new template and different peptides. We seem to have a general method for making synthetic lipopeptides in homogeneous form.