Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography

✍ Scribed by Haruko Takechi; Yasuo Goto; Hajime Takahashi; Minoru Machida

• Publisher: Journal of Heterocyclic Chemistry
• Year: 2001
• Tongue: English
• Weight: 64 KB
• Volume: 38
• Category: Article
• ISSN: 0022-152X
• DOI: 10.1002/jhet.5570380202

✦ Synopsis

Abstract

4‐(7‐Diethylaminocoumarin‐3‐yl)benzeneisocyanate (DACB‐NCO) was synthesized as a new fluorescent derivatization reagent for alcohols for use in high‐performance liquid chromatography (hplc). Saturated alcohols (C~6~‐C~22~) were derivatized in good yields into the corresponding fluorescent DACB‐carbamic esters by treating with DACB‐NCO. The DACB‐carbamic esters of these alcohols were clearly separated on a reversed‐phase hplc column (Inertsil ODS‐2, mobile phase: methanol‐water, excitation wavelength 402 nm; emission wavelength 488 nm). The detection limit (S/N = 3) of cetyl alcohol, as a test compound, was 5 fmol/10 μl.