Synthesis of 2,5-dihydroxy[carboxy-14C]benzoic acid for electrochemical protein modification

✍ Scribed by Varghese John; Jonathan L. Vennerstrom; Thomas J. Holmes Jr.

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: French
• Weight: 165 KB
• Volume: 23
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580230308

✦ Synopsis

The synthesis o f 2,5-dihydroxy[carboxy-14C]benzoic a c i d ( g e n t i s i c a c i d ) i s described. The procedure u t i l i z e d [carboxy-14C]salicylic a c i d as a s t a r t i n g m a t e r i a l . S a l i c y l i c a c i d was converted t o g e n t i s i c a c i d using potassium p e r s u l f a t e hydroxylation. The m i x t u r e o f h y d r o x y l a t i o n products i n t h i s r e a c t i o n was e a s i l y separated by UV-visual i z e d q u a r t z column chromatography t o o b t a i n t h e d e s i r e d isomer i n h i g h p u r i t y ( o v e r a l l y i e l d o f 38%).