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Synthesis of 2-keto-L-gulonic acid from gluconic acid by co-immobilized Gluconobacter oxydans and Corynebacterium sp.

✍ Scribed by Ji Aiguo; Gao Peiji


Book ID
110224888
Publisher
Springer Netherlands
Year
1998
Tongue
English
Weight
144 KB
Volume
20
Category
Article
ISSN
0141-5492

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We isolated Gluconobacter oxydans T-100 that had an activity to produce 2-KLGA from D-sorbitol; however, the yield of 2-KLGA was quite insufficient. Therefore, enzymes involved in the biosynthesis of L- sorbosone and 2-KLGA, L-sorbose dehydrogenase (SDH) and L-sorbosone dehydrogenase (SNDH), respect

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## Abstract Cell‐free extracts of __Gluconobacter melanogenus__ cells grown in L‐sorbose‐containing media contained an enzyme system capable of converting L‐sorbose to 2‐keto‐L‐gulonic acid while cells grown in glycerol media did not. This inducible enzyme was located in the participate fraction of