Synthesis of 17α-[125I]iodoethynyl-4,6-androstadien-17β-OL-3-ONE, an active-site-directed photoaffinity radiolabel for androgen-binding proteins

✍ Scribed by N. Scott Mason; Howard E. Smith; Benjamin J. Danzo; Jeffrey A. Clanton

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: French
• Weight: 460 KB
• Volume: 31
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580311002

✦ Synopsis

Abstract

17α‐[^125^I]Iodoethynyl‐4,6‐androstadien‐17β‐ol‐3‐one with a specific radioactivity of 24 Ci/mmol was prepared as an active‐site‐directed photoaffinity radiolabel for androgen‐binding proteins. The iodinated steroid was formed by the silver nitrate‐catalyzed reaction of N‐[^125^I]iodosuccinimide and 17α‐ethynyl‐4,6‐androstadien‐17β‐ol‐3‐one in acetone. N‐[^125^I]Iodosuccinimide was prepared by reaction of silver succinimide with iodine‐125 in dioxane, the latter being formed by oxidation of sodium iodide‐125 with sodium nitrite‐nitric acid in water‐hexane.