Synthesis of [14,15,17,17,18,18-2H6]-leukotriene B4

✍ Scribed by Dimitrios Tsikas; Joachim Fauler; Jürgen C. Frölich

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: French
• Weight: 306 KB
• Volume: 31
• Category: Article
• ISSN: 0022-2135
• DOI: 10.1002/jlcr.2580310502

✦ Synopsis

Abstract

Incubation of [14,15,17,17,18,18‐^2^H~6~] leukotriene A~4~ obtained by hydrolysis of chiral [14,15,17,17,18,18‐^2^H~6~] leukotriene A~4~ methylester with human monocytes resulted in the formation of [14,15,17,17,18,18‐^2^H~6~] leukotriene B~4~ (d~6~‐LTB~4~) and its trans isomers 6‐trans‐d~6~‐LTB~4~ and 12‐epi‐6‐trans‐d~6~‐LTB~4~ in high isotopic purity (99.4 % ^2^H). d~6~‐LTB~4~ was separated from its trans‐isomers by reversed‐phase high‐performance liquid chromatography and identified by gas chromatography mass spectrometry. d~6~‐LTB~4~ is shown to be a useful internal standard for gas chromatographic mass spectrometric analysis of LTB~4~ in biological matrix.