Synthesis, Characterization, Kinetic Parameters, and Diagnostic Application of a Sensitive Colorimetric Substrate for β-Galactosidase (2-Chloro-4-Nitrophenyl-β-D-Galactopyranoside)

Chemical synthesis and kinetic character

Chemical synthesis and kinetic characterization of UDP-2-deoxy-d-lyxo-hexose(“UDP-2-deoxy-d-galactose”), a donor-substrate for β-(1 → 4)-d-galactosyltransferase

✍ Geeta Srivastava; Ole Hindsgaul; Monica M. Palcic 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 578 KB

## Bovine p-(1 + 4kgalactosyltransferase (GalT) transfers galactose from UDP-galactose to B-D-GlcpNAc-terminating oligosaccharides to produce N-acetyllactosamine sequences. We report here the chemical synthesis, structural characterization and enzymatic evaluation of the very labile UDP-Zdeoxy-D-l