Synthesis, Characterization and Preliminary Biological Evaluation of P(HPMA)-b-P(LLA) Copolymers: A New Type of Functional Biocompatible Block Copolymer
✍ Scribed by Matthias Barz; Florian K. Wolf; Fabiana Canal; Kaloian Koynov; Maria J. Vicent; Holger Frey; Rudolf Zentel
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 362 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1022-1336
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✦ Synopsis
Abstract
We describe a synthetic pathway to functional P(HPMA)‐b‐P(LLA) block copolymers. The synthesis relies on a combination of ring‐opening polymerization of L‐lactide, conversion into a chain transfer agent (CTA) for the RAFT polymerization of pentafluorophenyl methacrylate. A series of block copolymers was prepared that exhibited molecular weights $\overline M _{\rm n}$ ranging from 7 600 to 34 300 g · mol^−1^, with moderate PDI between 1.3 and 1.45. These reactive precursor polymers have been transformed into biocompatible P(HPMA)‐b‐P(LLA) copolymers and their fluorescently labeled derivatives by facile replacement of the pentafluorophenyl groups. The fluorescence label attached to this new type of a partially degradable amphiphilic block copolymer was used to study cellular uptake in human cervix adenocarcinoma (HeLa) cells as well as aggregation behavior by fluorescence correlation spectroscopy (FCS).
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