✦ LIBER ✦
Synthesis and Triple-Helix-Stabilization Properties of Branched Oligonucleotides Carrying 8-Aminoadenine Moieties
✍ Scribed by Anna Aviño; Marta G. Grimau; Miriam Frieden; Ramon Eritja
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- German
- Weight
- 195 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0018-019X
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✦ Synopsis
Abstract
The synthesis of several branched oligonucleotides, i.e., of the parallel hairpins 5–8 and the Y‐shaped 9 is described, together with their use in the formation of pyrimidine⋅pyrimidine⋅purine triple helices. Special attention was paid to the optimization of the assembly of the second strand from asymmetric branching molecules. The presence of 8‐aminoadenine moieties in the Watson____Crick purine strand and 2′‐O‐methyl‐RNA in the Hoogsteen pyrimidine strand produced strong stabilization of the triplex.