Synthesis and study of a molecularly imprinted polymer for specific solid-phase extraction of vinflunine and its metabolite from biological fluids

✍ Scribed by Claire Lopez; Bérengère Claude; Philippe Morin; Martine Pelissou; Richard Pena; Jean-Paul Max; Jean-Paul Ribet

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 218 KB
• Volume: 34
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201100015

✦ Synopsis

Abstract

A molecularly imprinted polymer (MIP) was synthesized in order to specifically extract vinflunine, an anticancer agent, and its metabolite (4‐O‐deacetylvinflunine) from bovine plasma and artificial urine by solid‐phase extraction (SPE). Vinorelbine, a non‐fluorinated analogue of vinflunine, was selected as a template for MIP synthesis. The selectivity of MIP versus the template (vinorelbine) and other alkaloids (catharanthine, vinblastine, vincristine, vinflunine and 4‐O‐deacetylvinflunine) was shown by a SPE protocol carried out with non‐aqueous samples. A second protocol was developed for aqueous samples with two consecutive washing steps (AcOH–NH~2~OH buffer (pH 7, I=10 mM)–MeOH mixture 95:5 v/v and ACN–AcOH mixture 99:1 v/v) and an elution step (MeOH–AcOH mixture 90:10 v/v). Thus, MIP‐SPE of bovine plasma brought high recoveries, 81 and 89% for vinflunine and its metabolite, respectively. This protocol was slightly modified for artificial urine samples in order to obtain a good MIP/NIP selectivity; furthermore, elution recoveries were 73 and 81% for vinflunine and its metabolite, respectively. Repeatability was assessed in both biological matrices and RSD (%) were inferior to 4%. The MIP also showed a suitable linearity (r^2^ superior to 0.99), between 0.25 and 10 μg/mL for plasma, and between 1 and 5 μg/mL for artificial urine.