Synthesis and spectroscopic characterization of insulin derivatives containing one or two poly(ethylene oxide) chains at specific positions
✍ Scribed by M. Ehrat; P. L. Luisi
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1983
- Tongue
- English
- Weight
- 255 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-3525
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✦ Synopsis
We describe a synthetic method aimed a t specifically coupling poly(ethy1ene oxide) (PEO) to proteins without altering their spectroscopic properties. To do so, we have first modified the alcohol end group of PEO to an acid end group (PEO-COOH). Coupling of PEO-COOH to Gly A' methylsulfonylethyloxycarbonyl (Msc) protected insulin yielded Nos1,NfBz9-(PE0)z-insulin. Conversely, coupling of PEO-COOH to insulin whose Gly A' and Lys B29 amino groups were protected with Msc yielded NoB1-PEO-insulin. The products were obtained in a spectroscopically pure form and characterized by uv and CD spectroscopy. Conformational and biological studies are in progress.