Synthesis and secretion of interphotoreceptor retinoid-binding protein (IRBP) by isolated normal and rd mouse retinal photoreceptor neurons in culture
✍ Scribed by L. E. Politi; L. Lee; B. Wiggert; G. Chader; R. Adler
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 995 KB
- Volume
- 141
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Cultures of dissociated retinal neurons and photoreceptors from homozygous wild-type, heterozygous rdl+ and homozygous rdird retinas have been used to investigate the capacity of isolated photoreceptor cells to synthesize and secrete the interphotoreceptor retinoid-binding protein (IRBP). Retinal cells were dissociated on postnatal day 2 and grown in chemically defined medium in the absence of glial and pigmented epithelial cells. Expression of IRBP immunoreactive materials in these cultures was cell type-specific and developmentally regulated. Thus increasing numbers of rod photoreceptor cells showed immunoreactivity during the first week in culture, whereas nonphotoreceptor cell types remained consistently negative. Photoreceptor immunoreactivity could be detected in permeated (detergent-treated) as well as in unpermeated preparations, the latter suggesting that some IRBP is associated with the photoreceptor cell surface. These