Synthesis and secretion of immunoglobulin G by lymphocytes from cultured mouse spleen cells is not affected by heat shock
✍ Scribed by David I. Rodenhiser; Burr G. Atkinson; Jack H. Jung
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 622 KB
- Volume
- 135
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Short-term hyperthermic episodes (in vivo and in vitro) alter gene exprctssion in mammalian lymphocytes, resulting in the enhanced synthesis o i a select group of polypeptides-the heat-shock proteins-and the depressed synthesis of many normally synthesizcd polypeptides. Such alterations could have profound implications to an individual if the appropriate functioning of lymphocytes within the immune response was compromised by a depression in immunoglobulin synthesis during naturally occurring periods of hyperthermia, such as fever. In the present study we asked if heat-shock affects the facultative synthesis and secretion of immunoglobulin G by cultured mouse lymphocytes. We found that the quantity of immunoglobulin G synthesized and secrcted by these cells is not affected by heat-shock treatments sufficient to induce the synthesis of heat-shock proteins.
Methods
Animals Male and female 6-12 wk old BALB/c mice used in this study were obtained from Canada Breeding farms, St. Constant, Charles River, Quebec and maintained in the Animal Care facilities of the