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Synthesis and Purification of Thermally Sensitive Oligomer−Enzyme Conjugates of Poly( N -isopropylacrylamide)−Trypsin

✍ Scribed by Ding, Zhongli; Chen, Guohua; Hoffman, Allan S.


Book ID
127350659
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
225 KB
Volume
7
Category
Article
ISSN
1043-1802

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Unusual properties of thermally sensitiv
✍ Ding, Zhongli ;Chen, Guohua ;Hoffman, Allan S. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 164 KB

Reversible soluble-insoluble oligomer-enzyme conjugates have been prepared by conjugating a thermally sensitive oligomer, poly(N-isopropylacrylamide) [poly-(NIPAAm)] to trypsin. The conjugates can catalyze enzymatic reactions in solution and then may be separated from the solution by thermal precipi