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Synthesis and preliminary characterization of a new photoaffinity labeled adenosine nucleotide

✍ Scribed by Shan S. Wong; Danuta Glowacka; Dennis R. Johnson

Book ID
102372823
Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
343 KB
Volume
28
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

4‐Azido‐2‐nitrophenyl adenosyl pyrophosphate (ANAP), a new photoaffinity labeled adenosine nucleotide, was synthesized by coupling 4‐azido‐2‐nitrophenyl phosphate and adenosine 5′‐monophosphomorpholidate. Radioactive analogues were prepared from either [^14^C]‐AMP or iodination of 4‐azido‐2‐nitrophenol. ANAP was shown to be a competitive inhibitor of glucose‐6‐phosphate dehydrogenase against NADP with an inhibition constant of 9.5 μM. Iodination of ANAP did not affect its inhibition. Thus ANAP is a good photoaffinity analogue of NADP. It probably will also serve as an analogue of ATP or ADP.

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