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Synthesis and photochemistry of two cleavable heterobifunctional benzophenone protein crosslinkers

✍ Scribed by John E. Oatis Jr; Daniel R. Knapp


Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
247 KB
Volume
39
Category
Article
ISSN
0040-4039

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✦ Synopsis


Two new heterobifunctional protein crosslinkers were synthesized. These reagents contain the cysteine reactive maleimido group connected via a cleavable ester linkage to the benzophenone photoactivatable group.


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Synthesis of a new photo cross-linking agent incorporating chromogenicity, cleavability, and water solubility is described. The high efficiency of nitrene insertion observed upon photolysis of perfluoroaryl azides into organic solvents and proteins is extended to the design and synthesis of new mult