Synthesis and immobilization of a novel acridine derivative on microparticulate silica : A study of its interactions with single-stranded oligonucleotides by high-performance liquid chromatography
✍ Scribed by Rainer Bischoff; Fred E. Regnier
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 933 KB
- Volume
- 397
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
A novel approach for immobilizing acridine on 5-pm silica gel is described. The acridine moiety is functional&d with a carboxylic acid group at its reactive 9position and activated, leading to 9-acridinylpropionic acid N-hydroxysuccinimide ester. This derivative is efficiently bound to the silica matrix through a primary aliphatic amine group at the end of a fifteen-atom spacer arm. The chromatographic properties of the final stationary phases, as evaluated with d(T)rO and d(A),,-, at various pH values and organic solvent concentrations, resemble those of hydrophobic weak anion exchangers. When a secondary amine group is placed close to the acridine moiety in one of the packings, enhanced binding of the oligodeoxyribonucleotides is observed that goes beyond a purely additive effect.