Synthesis and hybridization studies of urea and carbamate linked thymidine dimers incorporated into oligodeoxynucleosides

✍ Scribed by Kristina M.K. Kutterer; George Just

• Book ID: 103982786
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 315 KB
• Volume: 4
• Category: Article
• ISSN: 0960-894X
• DOI: 10.1016/0960-894x(94)80011-1

✦ Synopsis

An efficient synthesis of urea, N-methyl urea and catbamate linked thymidine dimers has been accomplished. Incorporation of the dimers one or three times into a DNA oligonucleotide. via solid-phase synthesis, was performed. Thermal denatmation studies indicated that the urea-linked 12-mers selectively bind complementary DNA, whereas, the carbamate-linked oligomers bind poorly to both DNA and RNA.