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Synthesis and Evaluation of Chromogenic and Fluorogenic Analogs of Glycerol for Enzyme Assays

✍ Scribed by Eva Maria González-García; Johann Grognux; Denis Wahler; Jean-Louis Reymond

Publisher
John Wiley and Sons
Year
2003
Tongue
German
Weight
160 KB
Volume
86
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

The branched glycerol analogs 1 and 2 were prepared. Mono‐ester derivatives of these triols undergo a chromogenic or fluorogenic reaction in the presence of NaIO~4~. In contrast, both the diesters and the triols are themselves not chromogenic or fluorogenic. Diester derivatives of these triols can be used as probes for lipases. The tris‐phosphate derivative of 1 is a fluorogenic substrate for various phosphatases.

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