Synthesis and DNA-Cleavage Activities of Dinuclear Copper(II) Complexes of Urea-Bridged Macrocyclic Polyamines

✍ Scribed by Yang Yue; Jing Li; Ji Zhang; Zhong-Wei Zhang; Hong-Hui Lin; Shan-Yong Chen; Xiao-Qi Yu

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 395 KB
• Volume: 6
• Category: Article
• ISSN: 1612-1872
• DOI: 10.1002/cbdv.200800305

✦ Synopsis

Two dinuclear macrocyclic polyamine copper(II) (Cu II ) complexes, which have two cyclen units linked by urea, were synthesized as DNA-cleavage agents. The structures of these new dinuclear complexes were identified by HR-ESI-MS and IR analyses. The catalytic activities of DNA cleavage of these dinuclear Cu II complexes were subsequently studied. The results show that 6a was the better catalyst in the DNA-cleavage process than 6b. The effects of reaction time and concentration of complexes were also investigated. The results indicate, that the Cu II complexes could catalyze the cleavage of supercoiled DNA (pUC 19 plasmid DNA; Form I) under physiological conditions to produce selectively nicked DNA (Form II; no Form III was produced) with high yields (nearly 100%) in short time in the absence of reductant or oxidant.