Synthesis and decay of λ DNA replication proteins in minicells
✍ Scribed by Barbara Lipinska; Anna Podhajska; Karol Taylor
- Book ID
- 118315161
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 530 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0006-291X
No coin nor oath required. For personal study only.
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The purified bacteriophage 2 replication proteins O and P sediment separately in metrizamide gradients of low ionic strength as dimers. Together they interact with each other forming an oligomer, composed of two molecules of 20 and one molecule of ,~P. The 20-P oligomer is active in the in vitro rep
Minicells derived from E. coli x796(F+) are refractory to infection by phage M 13. However, after infection of the minicell-producing strain with M 13, phage DNA is found to segregate efficiently into newly formed minicells. The M 13 specific DNA present in minicells isolated several hours after inf