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Synthesis and circular dichroism study of the human salivary proline-rich protein IB7

✍ Scribed by Cécile Simon; Isabelle Pianet; Dr Erick J. Dufourc

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
111 KB
Volume
9
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

The solid phase synthesis of a 59 amino acid human salivary protein IB7 has been accomplished using Fmoc strategy. Because the protein contains 25 proline, 13 glycine and 9 glutamine residues the coupling time, piperidine delivery and acetic anhydride reaction time were increased. Yield after HPLC purification was 35%. Circular dichroism studies revealed that about one third of IB7 residues adopted a type II helix secondary structure, as found in collagen helices. The rest of the sequence adopts a random coil secondary structure. Copyright © 2003 European Peptide Society and John Wiley & Sons, Ltd.

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