𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Synthesis and characterization of DNA quadruplexes containing T-tetrads formed by bunch-oligonucleotides

✍ Scribed by Giorgia Oliviero; Jussara Amato; Nicola Borbone; Aldo Galeone; Michela Varra; Gennaro Piccialli; Luciano Mayol

Publisher
Wiley (John Wiley & Sons)
Year
2006
Tongue
English
Weight
360 KB
Volume
81
Category
Article
ISSN
0006-3525

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The solid phase syntheses of the bunch oligonucleotides 1 and 2 based on the sequences of the natural oligodeoxynucleotides (ODNs) d(TG~2~TG~2~C) (3) and d(CG~2~TG~2~T) (4), respectively, attached to a non‐nucleotidic tetrabranched linker, are reported. Bunch‐ODNs 1 and 2 were shown to form more stable monomolecular parallel G‐quadruplexes I and II when compared with their tetramolecular counterparts [d(TG~2~TG~2~C)]~4~ (III) and [d(CG~2~TG~2~T)]~4~ (IV), respectively. The structure and stability of all the synthesized complexes have been investigated by circular dichroism (CD), CD thermal denaturation experiments, and ^1^H‐NMR (nuclear magnetic resonance) experiments at variable temperatures. Particularly, the spectroscopic data confirmed that 1 adopts a T‐tetrad containing parallel‐stranded quadruplex structure I as in the tetramolecular complex III. © 2005 Wiley Periodicals, Inc. Biopolymers 81: 194–201, 2006

This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at [email protected]

📜 SIMILAR VOLUMES

Synthesis and Structural Characterizatio
Synthesis and Structural Characterization of PNA-DNA Quadruplex-Forming Chimeras
✍ Veronica Esposito; Antonio Randazzo; Anna Messere; Aldo Galeone; Luigi Petraccon 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 321 KB

## Abstract The synthesis and structural characterization of four PNA‐DNA chimeras − ^5′^TGGG^3′^‐t (**C1**), ^5′^TGG^3′^‐gt (**C2**), t‐^5′^GGGT^3′^ (**C3**) and tg‐^5′^GGT^3′^ (**C4**), where lower and upper case letters indicate PNA and DNA residues, respectively − are reported. The oligomers we