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Synthesis and characterization of DNA-functionalized calcium phosphate nanoparticles

✍ Scribed by V. Sokolova; O. Prymak; W. Meyer-Zaika; H. Cölfen; H. Rehage; A. Shukla; M. Epple

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
154 KB
Volume
37
Category
Article
ISSN
0933-5137

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✦ Synopsis

Abstract

Calcium phosphate nanoparticles were prepared by precipitation from aqueous solution. A stable colloid was formed by coating with DNA. The properties of the colloidal dispersion can be adjusted by variation of the inorganic nanoparticles. In particular, the partial substitution of calcium by magnesium or aluminum led to stable dispersions of nanoparticles. By a multi‐step precipitation process, it is also possible to include DNA into the particles in order to protect it from intracellular biochemical degradation. The dispersions were analyzed by dynamic light scattering, zeta potential measurements, analytical ultracentrifugation, and transmission electron microscopy.

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