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Synthesis and characterization of a new biotinylated gramicidin

✍ Scribed by Emmanuelle Suarez; Emmanuelle De; Gerard Molle; René Lazaro; Philippe Viallefont

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
115 KB
Volume
4
Category
Article
ISSN
1075-2617

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✦ Synopsis

A new linear gramicidin analog bearing a biotinyl group grafted on C-terminal part was designed to study ligand-receptor interactions. The C-terminal alcohol in the native peptide was first replaced by an amino group. Then the peptide was synthesized on a polystyrene resin functionalized by the 2-chlorotrityl chloride following a biotinylation performed in solution. This new N%-biotinyl-(EDA) 15 -Gramicidin A was reconstituted in planar lipid bilayers and exhibited channel activities similar to those of natural gramicidin, with unitary conductance value about 30 ps in 1 M KCl. Furthermore this ionophore activity was quenched by addition of streptavidin in the surrounding medium. Our system is an outstanding tool for monitoring ligand-receptor interactions and could be used for designing a new biosensor.

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