✦ LIBER ✦
Synthesis and binding studies of the 116-mer peptide containing the double cysteine-rich motifs of protein kinase C gamma
✍ Scribed by Hiroyuki Fukuda; Kazuhiro Irie; Akifumi Nakahara; Kentaro Oie; Hajime Ohigashi; Paul A Wender
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- French
- Weight
- 259 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0040-4039
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✦ Synopsis
The 116-mer peptlde contaaning the double cysteine-nch motifs of mouse protein kinase Cy (y-C 1A-C IB) has been synthesized using an Fmoc-solid phase strategy with a stepwise chain elongation. The peptide was purified only by reversed phase HPLC and gave satisfactory mass data (MALDI-TOF-MS and ESI-TOF-MS). Scatchard analysis of the zinc-folded pepUde revealed two binding sites of distinct affinities (Kd = 6.0 and 47.0 nM) comparable to those reported by Quest and Bell for GST-y-C 1A-C 1B fusion protein prepared by DNA recombination.