Synthesis, Analysis, Purification, and Intracellular Delivery of Peptide Nucleic Acids
β Scribed by Dwaine A. Braasch; David R. Corey
- Book ID
- 115639246
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 267 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1046-2023
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π SIMILAR VOLUMES
Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by O-[benzotriazol-1-yl]-1,1,3,3-tetramethyluronium hexafluorophosphate in combination with in situ neutralization of the resin allowed efficient coupling of all four Boc-protected PNA
## Abstract Peptide nucleic acids (PNAs) are DNA mimics with a neutral peptide backbone instead of the negatively charged sugar phosphates. PNAs exhibit several attractive features such as high chemical and thermal stability, resistance to enzymatic degradation, and stable binding to their RNA or D