Syntheses of poly[N-(2,2 dimethoxyethyl)-N-methyl acrylamide] for the immobilization of oligonucleotides

✍ Scribed by Laurent Veron; Marie-Caroline De Bignicourt; Thierry Delair; Christian Pichot; Bernard Mandrand

• Book ID: 102654925
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 791 KB
• Volume: 60
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/(sici)1097-4628(19960411)60:2<235::aid-app11>3.0.co;2-4

✦ Synopsis

Radical-initiated polymerization of N-( 2,2 dimethoxyethyl) -N-methylacrylamide has been carried out either in chloroform or methanol using 2,2'-azobisisobutyronitrile as an initiator, allowing us to prepare acetal containing water-soluble polymers. A kinetic study in both solvents showed that this monomer fairly homopolymerized (12,k;"' = 1 mol-'/' L'/' s-'/*). Static light scattering was used to characterize the molecular weight of these polymers. In addition, the Mark-Houwink-Sakurada relationship was established based on viscosity measurements performed at 25°C in water. Recovery of the aldehyde moieties on the polymer was achieved under mild conditions using a diluted inorganic solution. The analysis of the formation of aldehyde groups was performed by 'Hand '%-NMR. The covalent binding of oligodeoxyribonucleotides was carried out in water/acetonitrile mixtures with subsequent NaBH4 reduction of the imine bonds so as to stabilize the polymer/oligodeoxynucleotide conjugates.