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Syntheses of Novel Hapten–Protein Conjugates for Production of Highly Specific Antibodies to Formononetin, Daidzein and Genistein

✍ Scribed by Cyril Le Houérou; Catherine Bennetau-Pelissero; Valérie Lamothe; Françoise Le Menn; Pierre Babin; Bernard Bennetau

Publisher
Elsevier Science
Year
2000
Tongue
French
Weight
156 KB
Volume
56
Category
Article
ISSN
0040-4020

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✦ Synopsis

The syntheses of novel hapten-carrier protein conjugates are described. Isoflavones functionalised in C2 were coupled to bovine serum albumin or swine thyroglobulin. The antibodies obtained were used for the development of ELISAs (enzyme-linked immunosorbent assays) to measure the three isoflavones with IC 50 ranging from 15.6 to 100 ng/mL.

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