✦ LIBER ✦
Syntheses of deuterated leu-enkephalins and their use as internal standards for the quantification of leu-enkephalin by fast atom bombardment mass spectrometry
✍ Scribed by Dr. E. Benfenati; G. Icardi; S. Chen; R. Fanelli
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- French
- Weight
- 348 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0022-2135
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
We have developed a synthetic method for the preparation of di‐ and pentadeuterated leu‐enkephalin (LE), Tyr‐Gly‐Gly‐Phe‐Leu, by proton‐deuterium exchange using CF~3~COO^2^H. Four to six deuterium atoms are introduced using a reaction temperature of 120°C and if 5% of ^2^H~2~O is added the di‐deuterated LE is obtained. These deuterated compounds are used as internal standards to plot calibration curves of LE using fast atom bombardment mass spectrometry.