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Syntheses of 5-Chlorouracils/Thymines with 1-[Phosphono(Methyl/Difluoromethyl)]-1,2-Unsaturated-Moiety-Substituted Methyl Groups at N(1) and Human Thymidine Phosphorylase Inhibitory Activity

✍ Scribed by Matthew R. Birck; Keith Clinch; Graeme J. Gainsford; Vern L. Schramm; Peter C. Tyler


Book ID
102260229
Publisher
John Wiley and Sons
Year
2009
Tongue
German
Weight
269 KB
Volume
92
Category
Article
ISSN
0018-019X

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✦ Synopsis


Abstract

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By attaching (methyl)‐ or (difluoromethyl)‐phosphonate groups to the 1‐positions of ethene, cyclopentene or benzene, and attaching 1‐(methyl)‐5‐chlorouracil or 1‐(methyl)thymine groups to the corresponding 2‐positions, compounds 15 were prepared as potential inhibitors of recombinant human thymidine phosphorylase (TP). The products were designed to mimic the interatomic distance (ca. 3.41 Å) between the incoming phosphate and leaving pyrimidine groups at the transition state for the putative S~N~2 mechanism of TP. Free rotation around the (unsaturated‐CH~2~)–pyrimidine bonds in 15 enabled a span of ca. 2.40–4.40 Å between the CH~2~ or CF~2~ C‐atoms in the phosphonates and N(1) of the pyrimidines to be covered. The products were found to be ineffective inhibitors, and some reasons for this are given.