Syntheis and mass spectral characterization of p-acrylamidodophenylmercury chloride for high-capacity affinity gel chromatography
✍ Scribed by Anthony E. Reeve; Leslie P. Johnson
- Book ID
- 104144179
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 231 KB
- Volume
- 177
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
Organomercurial
agarose has been used for the selective purification of sulfhydryl proteins14, sulfhydryl nucleotides5-7 and sulthydryl substituted ribonucleic acidss4. This aEnity matrix has a low organomercurial content and is therefore only suitable for the fractionation of small quantities of thiol-containing compounds. We report here the synthesis and characterization of p-acrylamidophenylmercury chloride (I), which may be polymerized to form a polyacrylamide matrix containing very high Ievels of covalently bound mercury.
The synthesis of mercury derivatives of methyl acrylamide has been reported previouslyg, however, neither details of the synthesis nor characterization of the product was given.
Synthesis of compound I is achieved by direct coupling of acrylyl chloride to p-aminophenylmercuric acetate in dimethylformamide.
EXPERIMENTAL
Reagents
Mercuric acetate and aniline (Class II) were obtained from Fisher Scientific (Pittsburgh, Pa., U.S.A.). Aniline was distilled prior to use. Acrylyl chloride (98%) was supplied by Polysciences (Warrington, Pa., U.S.A.). Deuterated benzene (99.9x, ds) was supplied by Stohler (Waltham, Mass., U.S.A.