Syngeneic sensitization of mouse lymphocytes on monolayers of thyroid epithelial Cells. II. T and B cell involvement in primary responses

✍ Scribed by Jeannine Charreire

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 608 KB
• Volume: 12
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830120511

✦ Synopsis

Abstract

Using a previously developed in vitro model of sensitization of lymphoid cells against monolayers of syngeneic thyroid epithelial cells, the T or B nature of the population involved in the primary response has been investigated. For this purpose normal good responder CBA strain mice were used. T cell participation was clearly demonstrated in two ways: first, by the loss of primary sensitization after T cell depletion accomplished using several different techniques (including in vivo and in vitro anti‐Thy‐1.2 treatment, hydrocortisone and cyclophosphamide injections) and second, by the high response (measured by tritiated thymidine uptake) of T cell‐enriched suspensions obtained either by nylon wool filtration or Ig‐anti‐Ig columns.